African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Diversity, phylogenetic relationship and antibacterial potential of Bifidobacterium species isolated from raw milk production chain in Abidjan (Côte d'Ivoire)

Sylvie Mireille Kouamé-Sina1,2*, Adjéhi Dadié2, Kohei Makita4, Delia Grace4, Marcellin Dje2, Bernard Taminiau3, Georges Daube3 and Bassirou Bonfoh1
  1Centre Suisse de Recherches Scientifiques en  Côte d’Ivoire, 01 BP 1303 Abidjan 01. Côte d’Ivoire. 2Laboratory of  Biotechnology and  Food Microbiology, Faculty of Food Sciences and Technology, University of Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire. 3Department of Food Sciences, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster, 20 bât .B43b- 4000 Liege Belgium. 4International Livestock Research Institute ILRI, Nairobi, P. O. Box 30709 Nairobi, Kenya.
Email: kouamesylviemireille@yahoo

  •  Accepted: 31 May 2011
  •  Published: 09 October 2011



The local dairy commodity, from farm to retail point, is informal and often escapes safety surveillance and  results  in high contamination of local milk by pathogens. The objective of this study was to determine the biodiversity of Bifidobacterium species in the informal dairy production chain in Abidjan and evaluate their potential antibacterial activity against pathogens. Bifidobacterium species were identified after sequencing of hsp60 genes. Results showed that Bifidobacterium were present in 9% of samples. Milkers' hands (14%) and cows’s udders (14%) were the most contaminated with Bifidobacterium. These isolates belong to five different species. Most Bifidobacterium isolated are Bifidobacteriumminimum (53%) and Bifidobacterium pseudolongum subsp. Globosum (24.4%). The other strains are composed of one strain of Bifidobacterium thermophilum, Bifidobacteriumthermacidophilum subsp. suis and Bifidobacterium magnum. The isolated Bifidobacteriumspecies  have antibacterial activities that are not related to bacteriocins production, but to organic acids production (65%), which exert in vitro inhibitory action against Listeria monocytogenesSalmonella hadar and Salmonella typhimuriumStaphylococcus aureus,Escherichia coli O27 and Escherichia coli O157H7. However, ensuring milk safety along the local milk production chain requires implementation of good hygiene practices together with adapted technology, such as fermentation.


Key words: Bifidobacterium, antibacterial, milk, pathogens, Abidjan.