African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Isolation and characterization of thermostable protease producing Bacillus pumilus from thermal spring in Jordan

  Zakaria Al-Qodah1,2*, Hala Daghistani3 and Kholoud Alananbeh4
  1Department of Chemical Engineering, Al-Balqaa Applied University, Marka, 11134, P. O. Box 340558, Amman, Jordan. 2Faculty of Engineering, Taibah University, Medina, Saudi Arabia. 3Al-Salt College for Humanitarian Sciences,  Al-Balqa Applied University, Salt, Jordan. 4Faculty of Science, Taibah University, Medina, Saudi Arabia.
Email: [email protected], [email protected]

  •  Published: 19 July 2013

Abstract

 

A protease-producing thermophilic bacterium designated Bacillus pumilus JT3 was isolated and identified from a hot spring in Jordan by biochemical tests. The growth and enzyme production in shake flask culture using different operational conditions were studied and observed in a relatively wide temperature range from 25 to 80°C and pH from 6.5 to 13, with an optimal temperature of 60°C and pH 8. The effect of different carbon and nitrogen sources was tested. In addition, the effect of some metal ions on the enzyme stability was investigated showing enhancement with some ions such as Mn2+, Mg2+ and Ca2+ and deactivation with others such as Fe2+, Fe3+ and Zn2+.

 

Key words: Bacillus pumilus, Jordan hot springs, thermophilic microorganisms, thermostable enzymes, thermostable protease.