African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Influences of some biotic and abiotic factors on protein production and as inducers of Fusarium wilt disease resistance in lupine (Lupinus albus L.)

Heba I. Mohamed2*, Saieda S. Abd El- Rahman1 and Mohamed M. Mazen1
1Plant Pathology Research Institute, Agri. Res. Center, Giza, Egypt. 2Dep. of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt.
Email: [email protected], [email protected]

  •  Accepted: 25 February 2013
  •  Published: 09 April 2013


Two bacterial species; Pseudomonas fluorescens and Pseudomonas putida and three different chemical compounds; copper sulphate (CuSO4), Indole butyric acid (IBA) and potassium chloride (KCl) were tested for their ability to induce resistance in lupine plants against wilt disease caused by Fusarium oxysporum f. sp. lupini. Treatment of seeds with the selected bacterial species and chemical compounds significantly reduced wilt disease incidence of Fusarium oxysporum f. sp. in Lupine under greenhouse conditions. Potassium chloride and Pseudomonas fluorescens were most effective. Sodium dodecyl sulphate-polyacrylamide gel electrophresis (SDS- PAGE) analysis of lupine seedlings revealed that seeds treated with biotic and abiotic inducers resulted in a rapid induction of different novel Pathogenesis-related protein (PR) in shoot and root of lupine seedlings upon infection with the pathogen. These new proteins were not detected in untreated healthy or infected controls. This study aimed to use bacterial species and chemical compounds to decrease Fusarium wilt disease.


Key words: Pseudomonas fluorescens, Pseudomonas putida, copper sulphate, Indole butyric acid, potassium chloride