African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Destruction of Giardia Lamblia by electrical treatment of infected tab water

Alireza Jamshidi Fard1*, Laila Poorsaadat2 and Sara Jamshidi Fard3
1Department of Physiology and Medical Physics, School of Medicine, Arak University of Medical Science, Arak, Iran. 2Vali-e-Asr General Hospital, Arak University of Medical Science, Arak, Iran. 3Vali-e-Asr General Hospital, Arak University of Medical Science, Arak, Iran.
Email: [email protected], [email protected]

  •  Accepted: 08 September 2013
  •  Published: 04 October 2013


Protozoan parasites are responsible for the majority of gastrointestinal infections and even drinking treated tap water was reported risk factor for sporadic giardiasis. Variety of technologies has been used for elimination of Giardia cysts as well as removing other microbial contaminants and particles. The cysts are usually identified by their indistinguishable morphology. We applied direct current (DC) electrical charges for 1 to 4 min, 10 mA or 12 V, and 15 mA or 18 V in a handmade electrolytic cell filled with a prepared 105 cysts per ml emulsion, to examine its effectiveness as cysticide. Fresh human stools with Giardia, stained with Eosin Gelblich, were used to prepare wet mounts. Samples were examined by conventional light microscopy for the presence of cysts. Temperature and pH of the emulsions were measured in all stages. DC electrical charges equal or more than 15 mA (18 V in our circuit), in 2 min are practically lethal for Giardia cysts found in human stools, in a watery base with this concentration. pH of the emulsions varied between 3.5 to 8.8 in 22-24.4°C, when the currents were applied. 5 min after closing the DC electrical circuit, the tank water was treated and could be used.
Key words: Giardia lamblia, water treatment, direct electrical currents, cysticides.