African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Biochemical characterization and identification of bacterial strains isolated from drinking water sources of Kohat, Pakistan

Tassadaq Hussain1, Aneela Roohi1, Shehzad Munir1, Iftikhar Ahmed2, Jafar Khan1, Veronique Edel-Hermann3, Kil Yong Kim4 and Muhammad Anees1*
1Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan. 2SSO, Plant Biotechnology Program, National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Center (NARC), PARC Road, Islamabad, Pakistan. 3INRA-Université de Bourgogne, UMR 1229 Microbiologie du sol et de l’environnement - CMSE, INRA, 17 rue Sully, BP 86510, 21065 Dijon, France. 4Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju, 500-757, Korea.
Email: [email protected]

  •  Accepted: 25 February 2013
  •  Published: 16 April 2013


As the pure drinking water is inevitable for good health, it is necessary to evaluate it for bacterial contamination. This study was conducted on the different drinking water sources of Kohat, a Northern-Western District of Pakistan. Sampling was done from different drinking water sources including tap water, tube well water, home-well, bore-well and springs. Physiochemical analyses including pH, temperature, turbidity, total dissolved solids and electrical conductivity showed that all water samples were within ranges of the values prescribed by the World Health Organization. A total of 79 bacteria isolated from different samples were characterized. Eighty two percent of the strains were Gram negative and 64% of the total Gram positive bacteria were spore forming. The physiological characterization showed that 30.4% of the total bacterial strains were obligate aerobes while the rest were facultative anaerobes. Biochemical characterization and identification depicted enormous bacterial diversity where sixteen genera could be tentatively identified. Identification of 24 of the strains was further validated by using API 20E kit. Furthermore, the selected strains were analyzed for pH, temperature optimization and NaCl tolerance. Pseudomonas sp were the most abundant bacteria followed byBacillus sp. Some of the coliform bacteria could also be identified which present a potential health hazard.


Key words: API 20E, bacterial diversity, water-borne pathogens.