African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Isolation and identification of oleaginous endophytic fungi

Jie Xie, Lingling Song, Xinran Li, Qian Wu, Chuan Wang, Hui Xu, Yi Cao* and Dairong Qiao*
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of life Science, Sichuan University, Chengdu, Sichuan 610065, China.
Email: [email protected]

  •  Accepted: 29 March 2013
  •  Published: 07 May 2013


Endophytic fungi are a rich source of novel organic compounds with interesting biological activities and a high level of biodiversity. A total of 76 endophytic fungus strains were isolated from 13 species of plants. Through a preliminary screening and fermentation assay, a fungi named ML-GEN.1 isolated from Strobilanthes cusia was found and the lipid content reached 59%. This is the first report of oleaginous microorganism which is isolated from S. cusia. ML-GEN.1 was identified as Fusarium sp.ML-GEN.1 through morphological and molecular methods. Similar to vegetable oils, the fatty acid composition of lipid from Fusarium sp. ML-GEN.1 contained oleic acid (41.66%), palmitic acid (23.26%), linoleic acid (19.18%), and the unsaturated fatty acids amounted to about 61%. In waste molasses fermentation, Fusarium sp. ML-GEN.1 accumulated lipid to 29% of biomass when various sugars in waste molasses were utilized as the carbon source. The biomass was 22.8 g/L, which was much higher than the original value (12.7 g/L).


Key words: Oleaginous endophytic fungibiodieselwaste molasses.