African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Evaluation of rotavirus and intestinal parasite infection in a paediatric population in West Cameroon: Clinical characteristics and virus genotyping

  Antonella De Donno1*, Pierre René Fotsing Kwetche2, Giovanni Gabutti3, Adele Idolo1, Alessandra Cavallaro3, Elisabeth Lysette Kouemeni2, Lorenzo Badia4, Elisa Vanino4, Lazare Kaptue2, Tiziana Grassi1 and Rosario Cultrera4
  1Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of  Salento, Italy. 2Université des Montagnes, Bangangté, Cameroon. 3Hygiene and Preventive Medicine, Department of Clinical and Experimental Medicine, University of Ferrara, Italy. 4Infectious Diseases, Department of Clinical and Experimental Medicine, University of Ferrara, Italy.
Email: [email protected]

  •  Accepted: 20 August 2013
  •  Published: 30 August 2013



The prevalence of Rotavirus (RV)-related gastroenteritis in Cameroon was monitored in this study, identifying the circulating G-P types, and the presence of co-infection with intestinal parasites. The stool samples were examined for rotaviruses using chromatographic and molecular techniques, and parasites were identified by macroscopic and microscopic examination. 38% of samples were RV positive. Data revealed the presence of three different G-genotypes (G1, G2 and G8) and three P-genotypes (P [8], P[6] and P[4]). The main G-P combination was G1+G2P[8]. Entamoeba histolytica was identified in 44.4% of stool samples. The G2P[8] genotype identified is an unusual strain with the G1P[8] or G2P[4] more common. Genotype G8 strains, also associated with animals, have been sporadically recovered from humans and have been considered for inclusion in bovine-human reassortant vaccine. Epidemiological RV strain surveillance should be continued in order to support RV vaccination campaigns. The results also show a high prevalence of intestinal parasite infections.


Key words: Gastroenteritis, rotavirus, G/P genotype, semi-nested multiplex PCR, intestinal parasites, Cameroon.