African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Physicochemical and antimicrobial properties of Tunisian honeys: Honey inhibited the motility of bacteria

  Besma Ayari1,2,*, Feten Abbassi2, Mohamed Ali Hammami1 and Ahmed Landoulsi1
  1Laboratoire de Biochimie et de de Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, Zarzouna 7021, Bizerte, Tunisia. 2Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Tunisia.
Email: [email protected], [email protected]

  •  Accepted: 28 July 2013
  •  Published: 09 August 2013



Honey is a sweet flavourful product which has been consumed as a high nutritive value food. The present study aimed to characterize 12 samples of honeys collected from the North of Tunisia in respect to their floral origins. pH, hydroxyl methyl furfural, moisture content, ash, acidity, electrical conductivity, reducing sugars, apparent sucrose and total phenolic contents, were the parameters analysed in each sampleAll honey samples analyzed were found to meet European Legislation Ec Directive (2001/110) for all parameters studied. Antimicrobial assays showed that most honey samples tested inhibited the proliferation of Escherichia coli and Salmonella typhimuriumand altered theflagella driven motility of these two bacteria tested in swarming assay. We have also demonstrated that the most important antibacterial activity was observed for the honey originated from eucalyptus flower.


Keys words: North Tunisia, Honey, antimicrobial effect, physicochemical parameters, bacterial motility.


E. coli, Esherichia coliG, gentamycin; HMF, hydroxymethylfurfural;MBC, minimal bactericidal concentration; MIC, minimal inhibitory concentration; S. typhi,Salmonella typhimurium.