African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Multiple drug resistance of enteropathogenic Escherichia coli isolated from children with diarrhea in Kashan, Iran

Motallebi Mitra1, Piroozmand Ahmad1, Rohani Mehdi1, Akbari Hosein2 and Khorshidi Ahmad1*
1Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I. R. Iran. 2Department of Biostatistics and Public Health, Faculty of Health, Kashan University of Medical Sciences Kashan, I. R. Iran.
Email: [email protected]

  •  Accepted: 19 July 2011
  •  Published: 30 September 2011


Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in infants and children in developing countries. However, recent studies have shown its prevalence in developed countries. The purpose of this study was to evaluate the multi-drug resistant (MDR) of EPEC isolated from children less than five years old with diarrhea hospitalized in Kashan Shahid Beheshti hospital during 2009-10. Stool samples were collected from 313 children less than five years old with diarrhea at Shahid Beheshti Hospital, Kashan, Iran, from November 2009 to May 2010. Specimens were examined by polymerase chain reaction (PCR) test to determine the eae gene for detection of EPEC pathotype. The isolated multi-drug resistance pattern of EPEC was evaluated by disk diffusion agar. The antimicrobial disks used were ampicillin, nalidixic acid, ceftriaxone, ciprofloxacin, cefalexin, ceftazidime, streptomycin and imipenem. Of all 178 E. coli cases 51 (28.6%) EPEC pathotypes were isolated. The prevalence of EPEC was higher in children less than one year old. MDR rate in EPEC was 70.6%.The exact application of antibiogram test is necessary before antibiotic prescription for complete treatment and prevention of diarrhea caused by multi-drug resistant agents.


Key words: Enteropathogenic Escherichia coli, multiple drugs resistant, children, diarrhea.


Abbreviations: AE, Attaching and effacing; Bfp, bundle-forming pilus; EAF, Escherichia coli adherence factor; EPEC, enteropathogenic E. coliLA, localized adherence; LEE,locus of enterocyte effacement; MDR, multi-drug resistance; PCR, polymerase chain reaction; eae, EPEC attaching and effacing; EMB, eosin methylene blue; TSI, triple sugar iron agar; DNA, deoxyribonucleic acid.