African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Potentials of two Trichoderma species as antagonistic agents against Colletotrichum destructivum of cowpea

O. A. Akinbode1* and T. Ikotun2
  1Institute of Agricultural Research and Training, I.A.R and T, Ibadan, Nigeria. 2Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria.
Email: [email protected]

  •  Accepted: 01 November 2010
  •  Published: 04 March 2011



Antagonistic potentials of two Trichoderma species that is Trichoderma harzianum andTrichoderma pseudokoningii against the pathogen, Colletotrichum destructivum of cowpea was examined in vitro. Each of the Trichoderma species was paired with the pathogen in 9 cm Petri dishes with Acidified Potato Dextrose Agar (APDA) as the growth medium. Pathogen and antagonists were paired using three methods of pairing. Mycelia radial growth of both the pathogen and the antagonists were taken. The two Trichodermaspecies significantly inhibited radial growth of the pathogen. Introduction of the antagonists before the pathogen gave the best growth inhibition of C. destructivum. The inhibitory effect of the two Trichoderma species significantly differed from each other at P ≤ 0.05. T. pseudokoningii had better inhibition of the mycelia growth of C. destructivum than T. harzianum. T. pseudokoningii and T. harzianum have good antagonistic potentials againstC. destructivum.


Key words: Colletotrichum destructivum, Trichoderma pseudokoningii, Trichoderma harzianum, antagonists, inhibition, pathogen.