African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Isolation and characterization of microsatellite loci for Rhyacionia leptotubula (Lepidoptera: Tortricidae)

Jia-Ying Zhu, Song Yang, Qin-Wen Li and Yong-He Li*
  Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, College of Forestry, Southwest Forestry University, Kunming 650224, China.
Email: [email protected]

  •  Accepted: 20 September 2011
  •  Published: 23 October 2011

Abstract

 

Microsatellite as one of the most popular molecular markers provides a useful tool for population genetic structure analysis in insects. But few microsatellite loci have been developed for Lepidoptera. Here we isolated and characterized 9 polymorphic microsatellite loci in Rhyacionia leptotubula (Lepidoptera: Tortricidae). The number of alleles of these loci ranged from 2 to 11. Observed and expected heterozygosities ranged from 0.0000 to 0.5294 and from 0.3905 to 0.8719, respectively. Seven loci show significant Hardy-Weinberg deviations. These loci could provide insight into population genetic structure and dispersal patterns of R. leptotubula.

 

Key words: Rhyacionia leptotubula, microsatellite, population genetics, Lepidoptera.