African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Investigating the role of Candida glabrata SLN1 gene in stress adaptation: In silico and molecular analysis

Karen D. Guzmán-González1, Berenice Parra-Ortega2,3, María A. Martínez-Rivera1, César Hernández-Rodríguez2, Néstor O. Pérez4 and Aída V. Rodríguez-Tovar1*
1Laboratorio de Micología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Prol. Carpio y Plan de Ayala S/N, 11340 México, D.F. México. 2Laboratorio de Microbiología General, Departamento de Microbiología, ENCB, IPN, Pról. Carpio y Plan de Ayala S/N, 11340 México, D.F. México. 3Centro de Diagnóstico y Vigilancia Epidemiológica del Distrito Federal, Instituto de Ciencia y Tecnología del Distrito Federal, 03100 México, D.F., México. 4Laboratorio de Biología Molecular, Probiomed, SA de CV, Cruce de Carreteras Acatzingo-Zumpahuacán S/N, 52400 Tenancingo, Edo. de México, México
Email: [email protected]

  •  Accepted: 12 November 2012
  •  Published: 05 February 2013


The orthologous gene to histidine kinase SLN1 of Saccharomyces cerevisiae was found in the Candida glabrata genome database; we performed both in silico and molecular analyses of this gene. A phylogenetic analysis confirmed the close relatedness between these orthologous genes. We found that C. glabrata Sln1p presents two functional domains: a histidine kinase domain, and a response regulator domain, both similar to S. cerevisiae. Eight possible nitrogen source-activated transcription-factor binding sites and one for Mns4p/Mns2p were found. Expression analyses by semi-quantitative RT-PCR revealed that this gene was down-regulated when the yeast was cultured under osmotic and oxidative stress conditions, different nitrogen sources and acidic pH; but in alkaline pH, the expression of the SLN1 gene was recovered after 6 h. Hence, our results suggested that the SLN1 gene is involved in adaptation to different environmental stress conditions, similarly to the orthologous genes present in S. cerevisiae and Candida albicans


Key words: Candida glabrata, histidine-kinase Sln1p, osmotic and oxidative stress