African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Genetic diversity of bovine viral diarrhoea virus in Beijing region, China from 2009 to 2010

Jun-Jie Zhang1,2, Chong Ma2, Xi-zhi Li 2, Ying-Xia Liu1, Kai Huang1 and Jie Cao1*
1College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District 100193, Beijing, P. R. China. 2Sanyuan Lvhe Dairy Farming Center, No.5 Deyu Road in Demaozhuang, Daxing District 100076, Beijing, P. R. China.
Email: [email protected]

  •  Accepted: 16 September 2013
  •  Published: 18 October 2013


The genetic diversity of bovine viral diarrhea viruses (BVDV) from Beijing region, China between 2009-2010, was investigated using a phylogenetic analysis of partial 5’untranslated region (5’UTR) nucleotide sequences from 61 BVDV positive samples, and for 13 selected samples, the Npro region was also investigated. All the sixty-one blood samples were collected from cattle that were BVDV persistently infected (PI) within a BVD eradication program on Beijing farms during 2009 and 2010. Phylogenetic analysis based on 5’UTR sequences indicated that all BVDV positive samples were of the BVDV-1 genotype, but were clustered into four different subtypes: sub-genotype 1b (n = 31), 1m (n = 24), 1c (n = 4) and 1d (n = 2). BVDV-1b and 1 m are the most prevalent sub-genotypes in Beijing. The results also demonstrate that in most herds, certain BVDV-1 sub-genotype predominates. It is the first time that BVDV-1d is reported in China with unclear origin. The result of this study will be useful in constructing an effective vaccination plan to further control BVDV in Beijing region.


Key words: bovine viral diarrhea viruses 1 (BVDV-1), 5’untranslated region (5’UTR), Npro, phylogenetic analysis.