African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Microbacterium arborescens AGSB sp. nov., isolated from the rhizosphere of sand dune plant, Ipomoea pes caprae

Aureen L Godinho* and Saroj Bhosle
Department of Microbiology, Goa University, Taleigao Plateau, Goa-403206, India.
Email: [email protected]

  •  Accepted: 08 October 2013
  •  Published: 14 November 2013

Abstract

Phenotypic and phylogenetic studies were performed for the facultative alkalophile from the rhizosphere of Ipomoea pes caprae, a plant growing on coastal sand dunes. The isolate was Gram positive and showed optimum growth at pH 10.5. Chemotaxonomic analysis revealed that the isolate contained type B1 peptidoglycans with L-lysine as the diamino acid; rhamnose and galactose were the cell wall sugars and belonged unambiguously to the genus Microbacterium. The major menaquinones were MK-11 and MK-12. The 16S rDNA sequence of the Microbacterium arborescens isolate was deposited in the GenBank with an accession number DQ287961. The phylogenetic and phenotypic distinctiveness of the strain indicates it as a novel Microbacterium sp., named as M. arborescens AGSB.

 

Key words: facultative alkaliphile, Microbacterium arborescens AGSB, Coastal sand dune vegetation, Ipomoea pes caprae, 16S rRNA sequencing.