African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5238

Full Length Research Paper

Aflatoxigenic potential of Aspergillus section Flavi isolated from maize seeds, in Burkina Faso

Hamidou COMPAORE
  • Hamidou COMPAORE
  • DTA/IRSAT/CNRST, 03 BP 7047 Ouagadougou 03, Burkina Faso.
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Serge SAMANDOULOUGOU
  • Serge SAMANDOULOUGOU
  • DTA/IRSAT/CNRST, 03 BP 7047 Ouagadougou 03, Burkina Faso.
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Fidèle W. TAPSOBA
  • Fidèle W. TAPSOBA
  • DTA/IRSAT/CNRST, 03 BP 7047 Ouagadougou 03, Burkina Faso.
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Alima BAMBARA
  • Alima BAMBARA
  • Département de Biochimie-Microbiologie, Laboratoire de Biochimie et d’Immunologie Appliquée (LaBIA), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Hissein RATONGUE
  • Hissein RATONGUE
  • Université Catholique de l’Afrique de l’Ouest, Unité Universitaire à Bobo-Dioulasso (UCAO/UUB), 1052 Bobo-Dioulasso, Burkina Faso.
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Ignace SAWADOGO
  • Ignace SAWADOGO
  • DTA/IRSAT/CNRST, 03 BP 7047 Ouagadougou 03, Burkina Faso.
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Donatien KABORE
  • Donatien KABORE
  • DTA/IRSAT/CNRST, 03 BP 7047 Ouagadougou 03, Burkina Faso.
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Pane B. OUATTARA-SOURABIE
  • Pane B. OUATTARA-SOURABIE
  • Laboratoire National de Santé Publique (LNSP), 09 BP 24 Ouagadougou 09, Burkina Faso (Kadiogo / Centre).
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Hagrétou SAWADOGO-LINGANI
  • Hagrétou SAWADOGO-LINGANI
  • DTA/IRSAT/CNRST, 03 BP 7047 Ouagadougou 03, Burkina Faso.
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  •  Received: 14 June 2021
  •  Accepted: 27 July 2021
  •  Published: 31 August 2021

Abstract

The frequency of occurrence and four principal kinds of aflatoxin concentration in maize seeds grown in Burkina Faso was investigated. Ten (10) samples collected, were analyzed by high performance liquid chromatography (HPLC) with post-column derivatisation after immunoaffinity column cleanup. Eight strains of Aspergillus section Flavi were previously isolated from these samples and cultivated on “Aspergillus flavus and parasiticus agar (AFPA)” to ascertain if they belong to A. flavus or A. parasiticus species. The qualitative ability of aflatoxin production was also previously performed by fluorescence emission under ultra violet light at 365 nm after four (4) days of incubation at 30 °C on Coconut Agar Medium (CAM). Results showed that 70% of samples were contaminated by aflatoxins. The levels ranged from 0.93 to 58.94 µg/kg. Samples M1 and M10 had high concentrations, 58.94 µg/kg and 70.73 µg/kg; whereas M4 and M5 had low concentrations from 1.68 to 0.93 µg/kg, respectively. In these samples, four were contaminated with aflatoxin B1 (AFB1) and aflatoxin G1 (AFG1), two with AFB1 and aflatoxin B2 (AFB2) and one (01) with AFB1 only. We notice that AFB1 was the most prevalent member of aflatoxins, and AFG2 was absent in all samples.

 

Key words: Maize, Aspergillus, aflatoxins, HPLC, Burkina Faso.