African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210


Applications of molecular biology and biotechnology in oil field microbial biodiversity research

Zhuang Wen1,2,4 and Shao Hong-Bo1,3*
1The CAS / Shandong Provincial Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (CAS), Yantai 264003, China. 2Zaozhuang Vocational College, Zaozhuang 277800, China. 3Institute for Life Sciences, Qingdao University of Science and Technology, Qingdao 266042, China. 4The Graduate University of Chinese Academy of Sciences (CAS), Beijing 100049, China
Email: [email protected]

  •  Accepted: 28 March 2011
  •  Published: 30 September 2011


In recent years, with more and more attention given to the study of microorganisms in oil fields, molecular biology and biotechnology methodology has been applied prosperously.This article described the most commonly-used molecular biology techniques and related biotechnological advances in oil fields research at present, which are based mainly on 16s rRNA, such as 16S rRNA sequencing, denaturant gradient gel electrophoresis (DGGE),terminal restriction fragment length polymorphism (T-RFLP), etc. The article also points out their advantages and disadvantages, respectively and introduces some of the micro-organisms, which are of largest concerns in the research.


Key words: Biotechnology, molecular biology techniques, oil field, 16s rRNA, microbial biodiversity, prospect.