African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Detection of methanotrophic endosymbionts in Sphagnum sp. originating from Moszne peat bog (East Poland)

Zofia StÄ™pniewska, Agnieszka Kuźniar*, Anna Pytlak and Jolanta Szymczycha
Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Ul. Konstantynow 1I,  20-708 Lublin, Poland.
Email: [email protected]

  •  Accepted: 15 February 2013
  •  Published: 09 April 2013


Wetlands are one of the major sources of atmospheric methane. However, not all methane produced by methanogens reaches the atmosphere as a substantial amount of CH4 (1 to 90%) is recycled in soil aerobic zone by methanotrophic bacteria. It was found that methane oxidation is also conducted by methanotrophic bacteria endosymbionts ofSphagnum mosses. The aims of the study were to examine Sphagnum-involved CH4oxidation in transition moor in Eastern Poland and the molecular studies of methanotrophic communities inhabiting Sphagnum recurvumThe identification of endosymbiotic methanotrophs was performed by polymerase chain reaction (PCR) andfluorescence in situ hybridization (FISH) techniques. The methanotrophic activity of submerged and non-submerged Sphagnum mosses was determined at the level of 7.6±0.1 and 2.5±0.1 mM CH4g-1DWday-1 for complete plants from pool and lawn mosses, respectively. The highest activity was found in the top plants parts. On the basis of genomic analyses, it was found that endosymbionts belonged to methanotrophs of type I and II. The spread of Sphagnum species in peat bogs and the contribution of methanotrophic endosymbionts to methane recycling, (very important in relation to expected climate warming), could be greatly important in predicting the methane fluxes from wetlands.


Key words: Methane oxidation, methanotrophs, Sphagnum moss, symbiosis.