African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Estimation of total volatile fatty acid (VFA) from total organic carbons (TOCs) assessment through in vitro fermentation of livestock feeds

Md. Mukhlesur Rahman1,2*, Mohamad Amran Mohd. Salleh1, Nasrin Sultana3, M. J. Kim4 and Chang Six Ra4*
1Institute of Advanced Technology, University Putra Malaysia, UPM 43400, Serdang, Selangor, Malaysia. 2Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. 3Department of Animal Science, University Putra Malaysia, UPM 43400, Serdang, Selangor, Malaysia. 4Department of Animal Life System, Kangwon National University, Chuncheon 200-701, South Korea.
Email: [email protected], [email protected]

  •  Accepted: 07 February 2013
  •  Published: 09 April 2013


In vitro fermentation of 32 available feedstuffs was performed to investigate the relationship between soluble total organic carbon (TOCs) and volatile fatty acids (VFA) production. Correlation between VFA and methane (CH4) emission was also investigated. For this purpose, a fermentation reactor was designed to collect liquor for estimating TOCs and VFA during in vitro fermentation. The results showed that the VFA production was proportional to TOCs produced during in vitro fermentation (y = 1.1169x, r2 = 0.86). Forages produced more acetate (A) than propionate (P) and the A:P ratio of energy, protein and forages were 1.77:1, 2.08:1 and 2.80:1, respectively. CH4 production of feeds was also found proportional to VFA produced during in vitro fermentation (y = 0.0508x, r2= 0.58). It might be stated from this in vitro study that TOCs production could be used as an indirect index for estimation of VFA of livestock feeds.


Key words: Livestock feed, in vitro fermentation, total organic carbons, volatile fatty acids.