African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

An improved method for the extraction of nematodes using iodixanol (OptiPrep Ô)

Dewang Deng1, Allan Zipf1, Y. Tilahun1, G.C.Sharma1, J. Jenkins2  and K. Lawrence3
1Department of Natural Resources and Environmental Science, Alabama A&M University, Normal, AL. 2USDA-ARS-CSRL, Mississippi State, MS 3Entomology and Plant Pathology, Auburn University, Auburn, AL
Email: [email protected]

  •  Accepted: 16 July 2008
  •  Published: 31 July 2008

Abstract

A new nematode extraction technique was established, which is based on an iso-osmotic density-gradient medium (OptiPrepTM). This technique resulted in significantly higher numbers of clean eggs and vermiform nematodes that retain higher viability (48.6%) than samples processed with the sucrose method (28.7%). Nematodes survived exposure to OptiPrepTM for 22 hours without significant mortality whereas all nematodes died in the sucrose medium. OptiPrepTM provided a suitable, non-toxic alternative to the traditional density gradient material for the isolation of nematodes. This technique is convenient and relatively simple, with the added benefit of yielding cleaner samples compared to traditional isolation techniques.

 

Key words: Nematode extraction, density gradient, nematology.