African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Bovine mastitis: Prevalence of bacterial pathogens and evaluation of early screening test

SAIDI R.1,2*, KHELEF D.3, and KAIDI R.4  
1Department of Agronomy, University Telidji Amar, P. O. Box 37G, 03000 Laghouat, Algeria. 2LBRA, University Saad Dahleb, Blida, Algeria. 3Laboratory of Animal Health and Production, National Higher Veterinary School of Algiers, BP 161 Hacène Badi, EL Harrach, Algiers, Algeria. 4Department of Veterinary Sciences, Laboratory of Biotechnology Related to Animal Breeding, University Saad Dahleb, P. O. Box 270, Blida, Algeria
Email: [email protected]

  •  Accepted: 25 February 2013
  •  Published: 26 February 2013


Mastitis is the most complex and costly disease of dairy cows occurring throughout the world. In Algeria, the disease is not well investigated. A cross-sectional study to elucidate its magnitude, distribution, bacterial causes, and to investigate the reliability of a test for early diagnosis of mastitis in cattle in Blida and Ain Defla governorates, central region of Algeria, was carried out from May to December 2011 in a total of 108 milking cows using California mastitis test, clinical inspection of udder and bacteriological analysis. After conducting california mastitis test (CMT) in farms, quarter-based milk samples were collected from 108 cows. Also, 50 positive samples with CMT were collected for bacterial culture. Based on CMT, 29.20% of quarters and 29.62% of cows had subclinical mastitis; the sensitivity of CMT to infections with all bacteria was 96%. Staphylococcus aureuswere the most common pathogens (40%). Based on the results of the current study, CMT has very acceptable sensitivity in diagnosis.


Key words: Bacteriology, CMT, milk, sensitivity, subclinical mastitis