African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

A new PCR strategy for multilocus sequence typing of methicillin-resistant Staphylococcus aureus

Ambrina Khatoon, Syed Faraz Hussain, Syed M. Shahid* and Abid Azhar
The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi-75270, Pakistan.
Email: [email protected]

  •  Accepted: 25 March 2013
  •  Published: 09 April 2013


A single PCR protocol for methicillin-resistant S. aureus (MRSA) housekeeping genes, carbamate kinase, shikimate dehydrogenase, glycerol kinase, guanylate kinase, phosphate acetyltransferase, triosephosphate isomerases, and acetyl coenzyme-A acetyltransferase for multilocus sequence typing was developed. This PCR strategy provides a simple, rapid and cost effective amplification of the multiple loci of MRSA, with better quality data.  


Key words: Methicillin-resistant Staphylococcus aureus, PCR, multilocus sequence typing.