African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Expression of haptoglobin in Trichosporon asahii induced skin infection and its immunoregulatory role in mice

Jun-Hong Ao, Zhen-feng Hao, Yong Liao, He Zhu, Rong-Ya Yang*
Institute of skin damage and repair, General Hospital of Beijing Military Command of PLA, Beijing 100700, China.
Email: [email protected]

  •  Accepted: 26 April 2013
  •  Published: 21 May 2013


To investigate the immunoregulatory role of haptoglobin (Hp) in the Trichosporon asahiiinduced skin infection. A total of 80 BALB/c mice were assigned into immunosuppression (IS) group and non-immunosuppression (non-IS) group. Mice in both groups were subcutaneously inoculated with 3.2 × 10cfu/ml T. asahii suspension. 0.5, 1, 3 and 5 d after inoculation, mice was sacrificed and skin lesion was collected. mRNA expression of Hp gene was detected by RT-PCR. Microarray was employed to investigate immune related gene expression profile. In both groups, mRNA expression of Hp was detected as 0.5, 1, 3 and 5 d after T. asahii infection, but the Hp expression in IS group was significantly higher than that in non-IS group (P<0.01). One day after T. asahii infection, Hp expression was significantly higher than that of 0.5, 3 and 5 d after infection (P<0.01). A total of 11 immune related differentially expressed genes were identified in the microarray assay and the gene encoding Hp protein was up-regulated. Hp involves in the T. asahiiinfection of skin.


Key words: Trichosporon asahii, haptoglobin, skin, infection