Chemical and bioassay-guided fractionation of the chloroform-methanol (1:1) extract ofPhomopsis sp. By254, an endophytic fungus residing in the root of Gossypium hirsutum, yielded three compounds. The structures of these compounds were elucidated by a combination of spectroscopic analysis (MS, 1D- and 2D-NMR) to be epoxycytochalasin H (1), cytochalasin N (2), and cytochalasin H (3). All the compounds were subjected to antimicrobial action against plant pathogenic fungi and plant pathogenic bacteria. As a result, compounds 1 to 3 significantly inhibited the growth of Sclerotinia sclerotiorum,Bipolaris maydis, Fusarium oxysporum, Botrytis cinerea, Bipolaris sorokiniana,Gaeumannomyces graminis var. tritici and Rhizoctonia cerealis, with IC50 value ranging from 0.1 to 50 μg/ml. However, no inhibition of them was observed against plant pathogenic bacteria.
Key words: Phomopsis sp., Gossypium hirsutum, metabolites, antifungal, antibacterial.
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