African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Antifungal metabolites from Phomopsis sp. By254, an endophytic fungus in Gossypium hirsutum

Jing Fu1, Yang Zhou1, Hai-Feng Li1, Yong-Hao Ye1,2* and Jian-Hua Guo1*
  1College of Plant Protection, Nanjing Agricultural University; Engineering Center of Bioresource Pesticide in Jiangsu Province; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education; Nanjing 210095, P. R. China. 2College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, P. R. China.
Email: [email protected], [email protected]

  •  Accepted: 19 April 2011
  •  Published: 18 May 2011



Chemical and bioassay-guided fractionation of the chloroform-methanol (1:1) extract ofPhomopsis sp. By254, an endophytic fungus residing in the root of Gossypium hirsutum, yielded three compounds. The structures of these compounds were elucidated by a combination of spectroscopic analysis (MS, 1D- and 2D-NMR) to be epoxycytochalasin H (1), cytochalasin N (2), and cytochalasin H (3). All the compounds were subjected to antimicrobial action against plant pathogenic fungi and plant pathogenic bacteria. As a result, compounds 1 to 3 significantly inhibited the growth of Sclerotinia sclerotiorum,Bipolaris maydis, Fusarium oxysporumBotrytis cinerea, Bipolaris sorokiniana,Gaeumannomyces graminis var. tritici and Rhizoctonia cerealis, with IC50 value ranging from 0.1 to 50 μg/ml. However, no inhibition of them was observed against plant pathogenic bacteria.


Key words: Phomopsis sp., Gossypium hirsutum, metabolites, antifungal, antibacterial.