African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Using phenotypic based approaches to compare Escherichia coli isolates from human, livestock, fish and environmental sources within the Lake Victoria basin of Kenya

Sifuna Anthony Wawire1,2*, Onyango Miruka David3, Namuyenga Nelson2 and Ayub Ofulla2
1Ministry of Fisheries Development P.O. Box 36 Kalokol, Kenya. 2Maseno University; School of Public Health and Community Development; Private Bag Maseno, Kenya. 3Maseno University; Zoology Department; Private Bag Maseno, Kenya.
Email: [email protected]

  •  Accepted: 20 September 2013
  •  Published: 18 October 2013


The study compares Escherichia coli recovered from human, fish products, domesticated animals and the environment within the Lake Victoria basin on the basis of their antimicrobial susceptibility profiles. A total of 134 E. coli isolates were isolated from the collected samples. 52.2% of the E. coli isolates were found to be resistant to at least one antibiotic. Isolates originating from fish and soil showed the highest levels of resistance (100%). Based on the discriminant analysis (DA), most of the fish isolates were misclassified into soil category, probably due to the groups displaying similar Multiple Antibiotic Resistance (MAR) profiles. On the other hand, human isolates had the highest score of 0.55. The findings suggest that soil may be an important source of bacterial contamination of fish. Similarly resistance to antibiotics is widely prevalent among human, environment and domesticated animals within the Lake Victoria basin.


Key words: Escherichia coli; Environment; Discriminate; Antimicrobial resistance.