Hepatitis B virus (HBV) is a well known agent of acute and chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Around 400 million people worldwide carry the virus including 4.5 million only in Pakistan. Very limited research work on HBV genotyping has been done so far in Pakistan and there is a high level of discrepancy and inconsistency among different group of researchers. The aim of present study was to determine the prevalent genotypes of HBV in three big cities Karachi, Rawalpindi and Islamabad, Pakistan. A total of 200 HBV DNA polymerase chain reaction (PCR) positive samples (135 male and 65 female) from three big cities of Pakistan were tested for genotyping by two step nested PCR. Genotype D was detected as the most prevalent (58.5%) genotype followed by mix genotype A and D (31.5%) and genotype A was only (10%). Phylogenetic tree of 19 reference sequences constructed by using Clustal X program shows that all belong to subgenotype D1.
Key words: Hepatitis B virus genotype, subgenotypes, HBV genotype D, Pakistan
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