African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) fingerprinting reveals intra-serotype variations among circulating Listeria monocytogenes strains

Zulema Ruiz-Bolivar1, Ana K. Carrascal-Camacho1, Magda C. Neuque-Rico1, Carolina Gutiérrez-Triviño2, María X. Rodríguez-Bocanegra2, Raúl A. Poutou-Piñales3* and  Salim Mattar4
1Laboratorio de Microbiología de Alimentos, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. Colombia. 2Unidad de Investigaciones Agropecuarias (UNIDIA), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. Colombia. 3Laboratorio de Biotecnología Aplicada Grupo de Biotecnología Ambiental e Industrial (GBAI), Facultad de Ciencias. Pontificia Universidad Javeriana, Bogotá, D.C. Colombia. 4Instituto de Investigaciones Biológicas del Trópico (IIBT), Facultad de Medicina Veterinaria, Universidad de Córdoba, Montería, Colombia.
Email: [email protected]

  •  Accepted: 19 May 2011
  •  Published: 04 July 2011


Forty-five presumptive Listeria monocytogenes isolates were confirmed by multiplexpolymerase chain reaction (PCR) and characterized for antimicrobial susceptibility and tolerance to commonly used disinfectants. Isolates were also serotyped by PCR and characterized by enterobacterial repetitive intergenic consensus ERIC-PCR fingerprinting. All of the isolates showed PCR products of 938 bp (genus) and of 750 bp (species). Antimicrobial susceptibility was 100% for ampicillin, amoxicillin/ clavulanic acid, vancomycin and chloramphenicol, whereas for trimethoprim/ sulfamethoxazole it was 98, azithromycin 96, erythromycin 91, tetracycline 82, penicillin 97.8 (2.2% no susceptible), ciprofloxacin 84.4, rifampin 64.4, meropenem 71.1 and clindamycin 22.2%, respectively. All the isolates were resistant to cephalosporins. 71% of the isolates showed a MIC ≤ 200 ppm/10 to15 min for sodium hypochlorite and 98% a MIC ≤ 1.5%/2 to15 min for Tego-51. 58% of isolates were serotyped as 4b/4d/4e, 16% as ½b/3b, 7% as ½a/3a, and 4% as ½c/3c. ERIC-PCR showed 28 polymorphic bands ranging from 100 to 2810 bp that did not cluster according to any phenotype. ERIC-PCR fingerprinting revealed intra-serotypic variations and proved that different L. monocytogenes strains were circulating in the country during the isolation period.


Key words: Listeria monocytogenes, molecular serotyping, antimicrobial susceptibility, disinfectant tolerance, enterobacterial repetitive intergenic consensus- polymerase chain reaction (ERIC-PCR).