African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Identification of extended-spectrum-beta-lactamases(ESBLs), metallo-beta-lactamases (MBLs), Amp-C and KPC ß-lactamases among Klebsiella pneumoniae isolated from adults and pediatric patients in Iran

Fatemeh Fallah1, Mojdeh Hakemi Vala2, Hossein Goudarzi2, Ali Hashemi2*, Arezou Taherpour3, Khadijeh Bigdeli Shamloo4, Fariba Haj Shirmohammadi4 and Maliheh Habibi2
1Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran. 4Taleghani Hospital,Tehran, Iran.
Email: [email protected]

  •  Accepted: 26 March 2013
  •  Published: 30 June 2013


Klebsiella pneumoniae is one of the major bacteria that cause acute infections. β-lactamases are main defensive mechanisms in bacteria against drugs. The aim of this study was to determine antibiotic resistance patterns and detection of extended-spectrum-beta-lactamase (ESBL), MBL, Amp-C and KPC β-lactamases among K. pneumoniae strains isolated from adults and infants. This descriptive study was done on 83 K. pneumoniae isolated from two hospitals. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and broth microdilution methods according to Clinical Laboratory Standards Institute (CLSI) guidelines. ESBLs, MBL, Amp-C and KPC producing strains were detected by phenotypic confirmatory test, combination disk diffusion test (CDDT), Amp-C detection kit and Modified Hodge test, respectively. From 83K. pneumoniae strains, 48 (57.5%), 3 (3.5%), 23 (28%) and 5 (6%) were ESBL, MBL, Amp-C and KPC positive, respectively. In this study, Fosfomycin and Tigecycline were more active than other antibiotics. The results demonstrated that incidence of ESBLs, Amp-C, MBLs and KPC was critical especially in infants. Therefore, detection of drug resistance patterns in K. pneumoniae and β-lactamases producing isolates is important for the prevention and control of these infections.


Key words: Klebsiella pneumoniae, infants, extended-spectrum-beta-lactamase (ESBL), Amp-C, MBLs, KPC.