African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Presence of Coxiella burnetii in blood serum and concentration of IgG in infected cows

Miodrag Radinović*, Stanko Boboš, Ivana Davidov, Mihajlo Erdeljan and Marija Pajić  
Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg D.Obradovića 8, 21000 Novi Sad, Serbia
Email: [email protected]

  •  Accepted: 19 December 2012
  •  Published: 05 February 2013

Abstract

Infection with Coxiella burnetii in cows usually runs without typical symptoms. If clinical symptoms occur, they are mainly related to reproduction disturbance such as abortion, reproduction disorders, and low vital offspring. The aim of the paper was to determine the relation between IgG concentration and the presence of C. burnetii in serum of seropositive cows. Experiment was conducted on group of cows serologically positive toC. burnetii. For serological examination, enzyme-linked immunosorbent assay (ELISA) method was used. PCR method was used to prove the presence of C. burnetii in serum. Radial immunodiffusion was used to determine concentration of IgG in serum. Presence of C. burnetii in serum was from 48.6 to 66.6% in different stages of lactation and the concentration of IgG was from 76.1 to 95.4 g/L in different stages of lactation. In the control group of cows from same farm, the concentration of IgG in blood serum was from 35.7 to 42.2 g/L.

 

Key words: Cow, Coxiella burnetii, serum, IgG