African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

The chloride ion been responsible for filament formation and inhibitory effect on cell division in Zymomonas mobilis 232B growth

  Xinhui Wang1,2,3, Yanling Jin1,3*, Yang Fang1,3, Yuhong Huang1,2,3, Guohua Zhang1,3, Xiaofeng Gao1,2,3 and Hai Zhao1,3
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, China. 3Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
Email: [email protected]

  •  Accepted: 07 April 2011
  •  Published: 09 December 2011



To elucidate respective effects of sodium and chloride ion on filament formation inZymomonas mobilis 232B culture induced by NaCl, the morphological changes of cell grown in medium containing 0.2 M (NH4)2SO4, Na+(as Na2SO4) and Cl-(as NH4Cl) were observed using a scanning electronic nicroscope (SEM). The occurrence of filament formation began as early as 4 h after exposuring to NaCl and Cl- and the length was approximate 10 to 15 μm after exposuring for 20 h, which was 3 to 5 fold longer compared to normal cell, while Na+ and (NH4)2SO4 treated cells kept normal morphology, indicating that filament formation in NaCl-inhibited Z. mobilis cultures is attributable to chloride ion. In the presence of 0.2 M chloride ion, increase in cell number stop almost immediately, whereas cells continued to elongate slowly, suggesting that chloride ion has a direct inhibitory effect on cell division.


Key words: Zymomonas mobilis, chloride ion, filament formation, cell division.