African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Characterization and fermentability of sucrose thermolysis oligosaccharides caramel by human intestinal microbiota

Zhonghe WANG1, Jun WANG2, Li FU2, Shujuan YU1* and Sen MA1  
1College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, Guangdong, China. 2Department of Biology, Hanshan Normal University, Chaozhou 521041, Guangdong, China
Email: [email protected]

  •  Accepted: 25 February 2013
  •  Published: 05 March 2013


Sucrose thermolysis oligosaccharides caramel (STOC) was prepared by thermal treatment (160°C) under vacuum of acidified sucrose using citric acid as caramelization catalyst. Total carbohydrate content was 59.95% in STOC, which was constituted by kestose, mono- and di-oligosaccharide. In addition to carbohydrates, STOC also contained structurally unknown melanoidins. STOC was further subjected to in vitrofermentation with human fecal microbiota. After 24 h of incubation with human fecal suspension, STOC was extensively degraded (84.3%). Moreover, the results of total cell counts, major bacterial groups and short-chain fatty acid analysis indicated that STOC were the preferred substrates for human fecal microbiota. Significant increases after 24 h occurred in the total bacterial cells, Bifidobacteria and Bacteroides-Prevotella group in the presence of STOC (p<0.01). The increase in amounts of acetic and propionic acid was consistent with the role of these bacteria in the degradation of STOC.


Key words: Caramelization, oligosaccharide, intestinal microbiota, short-chain fatty acid