African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Effects of lake water chemistry on bacterioplankton community structures of three lakes

Dayong Zhao1,2*, Ting Ma1, Jin Zeng3, Wenming Yan2, Jianqun Wang1, Rui Huang1and Meng Wang1
1College of Hydrology and Water Resources, Hohai University, Nanjing, 210098, China. 2State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing, 210098, China. 3State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.
Email: [email protected]

  •  Accepted: 23 August 2011
  •  Published: 23 November 2011



To investigate the effects of lake water chemistry on bacterioplankton community composition, three different sized lakes (Lake Taihu, Lake Zixia and Lake Pipa) were studied. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA, followed by clone library analysis was used to explore the bacterial community structure in lake water. Cluster analysis of DGGE patterns indicated that bacterioplankton community compositions within one lake were similar. Canonical correspondence analysis (CCA) was carried out to interpret the response of the bacterial community structure to water chemistry. Sampling lakes and total phosphorus were two variables found to significantly correlate with DGGE patterns. Clone library analysis results indicated that the bacterioplankton community composition of three lakes differed markedly, even at the phylum and subphylum levels. The results of the homologous and heterologous coverage curves analysis based on the LIBSHUFF program also showed significant differences among the three clone libraries.


Key words: Bacterioplankton community structures, denaturing gradient gel electrophoresis (DGGE), multivariate analysis and water chemistry.