African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Bioformulation Pseudomonas fluorescens SP007s against dirty panicle disease of rice

Sutruedee Prathuangwong1,2*, Dusit Athinuwat3, Wilawan Chuaboon1, Tiyakhon Chatnaparat1 and Natthiya Buensanteai4
1Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, 10900 Thailand. 2Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok, 10900 Thailand. 3Major of Organic Farming Management, Faculty of Science and Technology, Thammasat University, Pathumthani, 12121 Thailand. 4School of Crop Production Technology, Institute of Agriculture Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000 Thailand.
Email: [email protected]

  •  Accepted: 08 October 2013
  •  Published: 28 November 2013


Two-different carrier formulations, kaolin and talc-based products were developed with Pseudomonas fluorescens SP007s biocontrol agent. SP007s viability in different carriers stored at room temperature (28 to 33°C) slowly declined to approximately 46.2 and 61.0% after 12-month-old shelf life. The decreased population was first found in five and month months of storage for kaolin and talc-based formulations, respectively. Field experiment with 6-foliar spray intervals (1 × 108 cfu/ml) of SP007s was conducted against naturally-occurred dirty panicle disease caused by a multiplex fungus at Suphanburi. The two bioformulations significantly reduced pathogen colonization on rice panicle and exhibited the greatest yield that correlated with increased defense-related enzyme accumulation in treated plants, compared to 4-fungicide spray intervals and nontreated control. Protection of seeds collected from colonized and noncolonized plants of dirty panicle treated with bioformulations (1 × 106 cfu/ml) and fungicides (copper hydroxide) was further determined for 12 months of storage at room temperature. The best results in reducing 6-causal fungi including Alternaria padwickii, Cercospora oryzae, Curvularia lunata, Fusarium semitectum, Helminthosporium oryzaeqe and Sarocladium oryzae; and induced seedling vigor (35%) were obtained from SP007s kaolin-based formulation evaluated at 8-month storage, but not at 12 months which indicated that these causal pathogens totally recovered their colonization except S. oryzae. In 8-month trials, control efficacy with dose of 1 × 106 cfu/ml SP007s seed treatment, the increase in SP007s populations relatively with the decreased colonization of pathogens could be found. SP007s in kaolin-based formulation increased GABA in SP007s treated seeds suggesting this plant bioactivator may involve plant’s defense against stress conditions also.


Key words: Biocontrol, multiplex fungus causes, induced systemic resistance, protective enzymes, reduced chemical application.