African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Contamination of cattle carcasses by Escherichia coli shiga like toxin with high antimicrobials resistence

Everlon Cid Rigobelo1*, Renato Pariz Maluta2, Clarissa Araújo Borges2, Lívia Gerbasi Beraldo2, Manoel Victor Franco3, Lemos Sirlei Aparecida Maestá1 and Fernando Antonio de Ávila4
1Campus Experimental de Dracena- UNESP, Brazil. 2Programa de Pós-Graduação em Microbiologia Agropecuária UNESP Jaboticabal Brazil. 3Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal. Departamento de Biologia Aplicada a Agropecuária, Brazil. 4Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Departamento de Patologia Veterinária, Brazil.
Email: [email protected]

  •  Accepted: 15 March 2011
  •  Published: 18 August 2011


During processing of cattle carcasses, contamination may occurs with the transfer of microbiota of animals feaces to carcasses. This contamination many times may be byEscherichia coli carriers of virulence factor as stx and eae genes being classified as Shiga like toxin. Shiga toxin-producing Escherichia coli (STEC) is recognized wordwide as human pathogen. A survey was performed to determine the sensibility profile to several antimicrobial drugs of STEC in carcasses obtained from an abattoir in Brazil between March 2008 and August at 2009. A total of 120 STEC were isolated. All isolates were confirmed as being E. coli by their biochemical analysis and submitted to polymerase chain reaction (PCR) for detection of stxeae and ehly genes. No strains was isolated being carriers of ehly gene. The number of isolates carriers of eae gene were 48/120. The most frequent resistance was seen against cephalothin (84.0%), streptomycin (45.0%), nalidixic acid (42.0%) and tetracycline (20.0%). Multidrug resistance (MDR) to three or more antimicrobial agents was observed in 46 (38.3%) E. coli isolates. The findings of STEC and MRD show that cattle carcasses may be a reservoir of pathogenic bacterial for the consumer public.


Key words: Multi-drug resistance, Escherichia coli, shiga toxin-producing Escherichia coli(STEC).