African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Comparative analysis on the curing effect of acridine orange (mutagen) on beta - lactamase producing Staphylococus aureus, from bovine and human origin

ONYENWE, N. E.1,   MBATA T. I.2* and ADELEKE, O. E.1
  1Department of Pharmaceutical Microbiology, University of Ibadan, Oyo state, Nigeria. 2Department of Microbiology, Federal Polytechnic Nekede, Owerri, Nigeria.
Email: [email protected]

  •  Accepted: 17 October 2011
  •  Published: 09 November 2011



The effect of the mutagen (acridine orange) on the multi-resistant antibiotics beta lactamase producing Staphylococcus aureus strain of bovine and human origin was investigated and tested individually using iodiometric (cell supervision) method for the level of beta-lactamase produced by each strain and antibiotic – sensitivity screening using single disc agar diffusion method. Analyses revealed that 80% of the isolates from bovine origin produced beta-lactamase while only 20% showed absence of the enzyme, while 68% of the human isolates produced beta-lactamase while only 32% showed absence of the enzyme sensitivity to cefotaxime, cefuroxime and ceftriaxine were observed in both strains of bovine and human origin. At high concentrations of the mutagen (250  µg/ml), zones of inhibitions were low for penicillin, amoxicillin and augmentine at reference MIC’s of 0.25 µg/ml, while the cephalosporins recorded high zones of inhibition at 0.5 and 1.0 µg/ml reference MIC’s in all the strains at 250 and 200 µg/ml of the concentration of the mutagen (acridine orange). The resistance bovine strains producing beta-lactamase resisted curing with acridine orange than the human strains of S. aureus.


Key words: Acridine orange, Staphylococcus aureus, beta-lactamase, iodometric.