African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Molecular detection of bla TEM and bla SHV genes among clinical isolates of Escherichia coli from Kashan, Iran

Khorshidi Ahmad, Rohani Mehdi, Akbari Motahare and Motallebi Mitra*  
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran
Email: [email protected]

  •  Accepted: 02 January 2013
  •  Published: 26 February 2013


Escherichia spp. are opportunistic pathogens that cause nosocomial infections. Because of their acquisition of multi drug resistant plasmids, these organisms are resistant to a number of antibiotics, including extended spectrum cephalosporins and aminoglycosides. The aim of this study is to detect extended spectrum beta-lactamase (ESBL) producingEscherichia coli isolated from Shahid Beheshti Hospital in Kashan. This descriptive study was done on clinical specimens isolated from Shahid Beheshti Hospital in Kashan. Identification of the isolated bacteria was done by standard biochemical testes. Determination of antimicrobial susceptibility was done by disk diffusion method. The ESBL production was investigated on isolates by double disk synergy technique. ESBL producers were confirmed by MIC method. PCR amplification of ESBL genes, TEM-1 and SHV-1 was carried out. 150 specimens were E. coli and 70 were ESBL (46%). Of the total of 70 specimens isolates, 9 out of 40 (13%) included TEM-1 and SHV-1. 5 specimens (7%) were SHV-1 and 44 specimens (63%) were TEM-1. Given the high levels of resistance, accurate anti-biogram tests before prescribing antibiotics and avoiding indiscriminate use of antibiotics are essential.


Key words: B-lactamase, extended spectrum beta-lactamase (ESBL), Escherichia coli, nosocomial infections