In present study, a nucleopolyhedrovirus was isolated from the infected larvae ofDendrolimus kikuchii, which is a serious pest for a variety of conifers in China. Ultrastructural observation revealed that this virus is a multiple-nucleocapsid nucleopolyhedrovirus (MNPV), which was encapsulated within the envelope of each virion. Based on the morphological characteristics, it was identified as a new virus and named as DkNPV. It exhibited high potential as a biocontrol agent to protect the forest because of the high virulence against the third-instar larvae of D. kikuchii. The virus passage in the host after 4 generations, toxicity and ultrastructural morphology were stable.
Key words: Dendrolimus kikuchii, multiple-nucleocapsid nucleopolyhedrovirus,Dendrolimus Kikuchii Matsumura Nuclear Polyhedrosis Virus (DkNPV), virulence, ultrastructure, biological control.
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