African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Immobilization of cellulase on modified mesoporous silica shows improved thermal stability and reusability

Hailei Yin1*, Zhong-liang Su5, Hongbo Shao2,5*, Jinfang Cai1, Xiaofei Wang3 and Haixia Yin4
1Department of Trauma and Orthopaedics, General Hospital of Jinan Military Region, 25, Shi-Fan Rd, Jinan 250031/ No.401 Hospital of People Liberation Army, 22, Min-Jiang Rd, Qingdao 266071, China. 2Key Laboratory of Coastal Biology and Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China. 3Out-patient Department of Joint Logistics Department of the Jinan Military Region, 36, Ying Xiong Shan Rd, Jinan 250002, China. 4First Retired Cadres of Joint Logistics Department of the Jinan Military Region, 55, Yu-Han Rd, Jinan 250002, China 5College of Chemical Engineering/Institute of Life Sciences, Qingdao University of Science and Technology (QUST), Qingdao 266042, China.
Email: [email protected], [email protected]

  •  Accepted: 29 March 2013
  •  Published: 30 June 2013


Cellulase was covalently immobilized using modified mesoporous silica as carrier, and the characters of immobilized cellulase were investigated including the optimum pH, the optimum temperature, thermal stability, reusability, storage stability and enzyme kinetics. As compared to the free enzyme, the optimum pH of the immobilized cellulase showed no changes, the optimum temperature showed a slight increase to 60°C. The immobilized enzymes demonstrated enhanced thermal stability, while the free enzymes lost almost 40% initial activity, the immobilized forms lost only 9% initial activity within a period of 120 min at 60°C. The immobilized cellulase showed excellent reusability. After 11 cycles, the cellulase immobilized on modified mesoporous silica still retained 89% of its initial activity. At optimized conditions, Km and Vmax values for the immobilized enzyme were slightly increased when compared with those of free enzyme. The characters of cellulase immobilized on modified mesoporous silica suggest that the immobilized enzyme has high potential for application in the industrial degradation of cellulose.


Key words: Renewable energy, cellulose, immobilization, mesoporous silica, cellulose.