African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Prevalence, seasonality and antibiotic susceptibility of thermophilic Campylobacters in ceca and carcasses of poultry birds in the “live-bird market”

Saba F. D Parkar1, Dhara Sachdev2, Nadine  deSouza1, Aniket Kamble2, Gayatri Suresh1, Hitendra  Munot3, David Hanagal4, Yogesh Shouche3, Balu Kapadnis1
1Department of Microbiology, University of Pune, Ganeshkhind, Pune 411 007, India. 2Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411 007, India. 3National Centre for Cell Science (NCCS), University of Pune Campus, Pune, India. 4Department of Statistics, University of Pune, Ganeshkhind, Pune 411 007, India.
Email: [email protected]

  •  Accepted: 03 May 2013
  •  Published: 21 May 2013

Abstract

Thermophilic Campylobacter spp. are the primary cause of human diarrhea. The common source of infection is contaminated poultry. This study aimed to establish the prevalence of Campylobacter spp. in poultry obtained from ‘pluck shops’ and provide a baseline of resistance profiles of Campylobacter spp. isolates obtained. A biphasic approach, with qualitative detection as well as quantitative enumeration of Campylobacterspp. was used. We examined 240 samples each, of carcasses and cecum of poultry obtained from ‘pluck shops. Amongst the cecum samples and carcasses, 59.5 and 57%, respectively were positive for Campylobacter spp. The average Campylobacter spp.concentration was 2.69 (S.D 0.419) log10 CFU/mL and 4.55 (S.D 0.607) log10 CFU/g for carcass rinsate and cecum, respectively. Of the 225 Campylobacter isolates studied, 76.9% were identified as Campylobacter jejuni and 23.1% as Campylobacter coli. Susceptibilities for 112 strains of C. jejuni and 31 strains of C. coli were determined for 12 antibiotics by the agar diffusion technique. According to the minimal inhibitory concentration, a marked resistance to gentamycin and chloramphenicol was also demonstrated. According to the antibiotic resistance profiles, the isolates appeared to differ from each other.

 

Key words: Campylobacter, seasonality, antibiotic resistance, India, wet-market, pluck-shop