African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Ethanol production from the water hyacinth Eichhornia crassipes by yeast isolated from various hydrospheres

G. O Ogawa Masami, IshidaYukinari Usui and Naoto Urano*
Laboratory of Marine Biochemistry, Tokyo University of Marine Science and Technology, Konan, Minato, Tokyo, 108-8477, Japan.
Email: [email protected]

  •  Accepted: 18 December 2007
  •  Published: 31 May 2008


Water hyacinth is a promising plant for the production of bioethanol. The water hyacinth was saccharified with diluted sulfuric acid and fermented to ethanol by yeast obtained from different hydrospheres. We found that the best conditions for water hyacinth hydrolysis were 1% (v/v) sulfuric acid at 121oC for 1 h. In the next step, we searched for yeast that could produce ethanol from the water hyacinth hydrolysate. For this, 624 strains were isolated from 28 bodies of water. Strain 484 produced 22.4 ml of ethanol/kg of dried water hyacinth, which suggests that it may be an efficient producer of bioethanol. Through sequence analysis, we identified strain 484 as Candida intermedia.


Key words: Biomass, ethanol, fermentation, hydrospheres, water hyacinth, yeast.