African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

A novel Alcaligenes faecalis antibacterial-producing strain isolated from a Moroccan tannery waste

Ilham Zahir*, Abdellah Houari, Wifak Bahafid, Mohammed Iraqui and Saad Ibnsouda
Laboratory of Microbial Biotechnology, Department of Biology, Faculty of Sciences and Technology, University Sidi Mohamed Ben Abdellah, BP 2202, Road of Immouzer, Fez, Morocco.
Email: [email protected]

  •  Accepted: 06 November 2013
  •  Published: 28 November 2013


Drug resistance of many harmful bacteria still represents a real public health problem. This is why the development of new bioactive substance becomes urgent. To reach our goal, a screening of bioactive substance produced by microorganism, was undertaken. In this work, we reported data on a bacterial strain isolated from the tannery of Fez (Morocco) that showed a broad antagonistic effect against a group of Gram-negative and positive bacteria especially Mycobacterium smegmatis. The antibacterial compounds producer, BW1 was identified as Alcaligenes faecalis on the basis of phenotypic characteristics, biochemical analyses and PCR amplification of 16 S ribosomal RNA gene followed by sequencing (100% of homology). The antibacterial compounds were synthesized during the exponential growth phase of A. faecalis. The antibacterial compounds were not affected following heat treatment and protolytic enzymes that indicated the non-proteinaceous nature of the active agents. The bacterial extract was assayed for cellular toxicity to fresh human erythrocytes and found to have no hemolytic effect.


Key words: Bacterial resistance, antibacterial compounds, Alcaligenes faecalis.