Abstract

Ibuprofen (IBU) and Diclofenac (DIC) are non-steroidal anti-inflammatory drug which have shown to have broad antimicrobial activity and limit biofilm formation. Aim of this study was to evaluate the effects of IBU and DIC against biofilm formation strong strains Escherichia coli and Klebsiella pneumoniae associated with urinary tract infections (UTIs). Additionally some virulence factors, antibiotic resistance, ESBL production were estimated to find correlation with the biofilm production and acil homoserine lactone (AHL). Our results suggest that IBU and DIC, could be useful in the treatment of urinary tract infections caused by E. coli and K. pneumoniae. IBU with CMIB 8, 30 and 125 mg/L, caused dramatic reductions in some cases and inhibited virulence factors which changes in the morphotypes expressed on congo red agar. DIC with CMIB 30 and 50 mg/L caused reductions in biofilm formation 50.1%. In this study, were evidenced the relationship between virulence factors, production of AHL and multi drugs resistance with biofilm production.

Key words: Diclofenac, ibuprofen, biofilm, acil homoserine lactone (AHL), Escherichia coli, Klebsiella pneumoniae.