African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Evaluation of specimens in which the urine sediment analysis was conducted by full-automatic systems and a manual method together with urine culture results

Okhan Kadir AKIN1, Muhittin A. SERDAR2*, Zeynep CIZMECI3 and Ozlem GENC3
1Kecioren Research and Training Hospital, Clinical Chemistry and Laboratory Science, Ankara, 06380, Turkey. 2Gulhane School of Medicine, Department of Clinical Chemistry, Ankara, 06018, Turkey. 3Kecioren Research and Training Hospital, Microbiology and Clinical Microbiology, Ankara, 06380, Turkey.
Email: [email protected]

  •  Accepted: 15 July 2011
  •  Published: 04 August 2011


The aim of the present study is to assess leukocyte and bacterial counts, and urine strip leukocyte esterase and as well as nitrite results from IQ200 and UriSed full-automatic urine sediment analyzers, with urine culture results. Six hundred urine cultures were performed in accordance with the routine laboratory procedures of the hospital. A growth <104 CFU/mL in the culture was considered negative. After the inoculation of urine samples into the culture media, urine samples were analyzed by IQ200 and UriSed full-automatic urine analyzers, respectively. As ≥104 CFU/mL are considered the diagnostic criterion for UTIs, 39 (6.5%, n=600) urine specimens yielded positive cultures. The positive likelihood ratios (PLR) of the IQ200 were suitable for bacteria and nitrite measurements (PLR>10); however, negative likelihood ratios (NLR) were inadequate for both devices and the manual method (NLR >0.3 for all). However, when the utilization of two tests in combination was analyzed, the diagnostic odds ratio was 48 for the IQ200 (95% confidence interval CI, 12.1-190) and 168 for the UriSed (95% CI, 20.6-1369) in cases in which leukocyte, bacteria, and nitrite measurements were high together. The results of the IQ200, UriSed, and urine strip test do not accurately reflect the urine culture results. Nevertheless, it was concluded that negative nitrite and leukocyte results of both automated and manual tests could be utilized in ruling out a urinary tract infection (UTI) diagnosis.


Key words: IQ200, UriSed, KOVA® system, full-automatic urine sediment analysis, bacteriologic urine culture, urine microscopy