The effect of Avid® on the induction of synnema-like structure of Aspergillus flavus (61 strains) grown on various types of agar media was investigated. Other related species of Aspergillus within the section Flavi, A. parasiticus (14 strains), A. nomius (14 strains), A. psuedotamarii (2 strains),A. tamarii (20 strains) and A. oryzae (5 strains), were also evaluated for comparison. When Avid®was added into five different solid media (Czapek, V8, Aspergillus flavus and A. parasiticus, corn meal and potato dextrose agar) at 0.004% (v/v), only the A. flavus strains (all 61 tested) were able to produce synnema-like structures, and only in the Czapek agar medium containing Avid®. The effect of carbon (sucrose, glucose and starch) and nitrogen (NaNO3, urea and peptone) sources in Czapek medium containing Avid® on the induction of a synnema-like structure was also determined, with synnema-like structure induction being found in all three carbon sources but only in NaNO3 as the nitrogen source. All the three of the inert solvents in Avid® (mineral oil, butylated hydroxytoluene and n-methylpyrrolidone) did not induce synnema-like structure formation, indicating that abamectin, an active ingredient of Avid® together with NaNO3 likely play important roles in the induction of synnema-like structure of A. flavus.
Key words: Aspergillus flavus, Avid®, synnema-like structure.
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