African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Role of autophagy in inhibition of brucella-containing vacuole-lysosome fusion in murine macrophages

Fei Guo1, Zhen Li1, Chuangfu Chen1*, Yuanzhi Wang3, Hui Zhang 2, Penyan Wang1, Jun Qiao1, Yong Wang1, Ke Zhang1 and Guoqing Du1
  1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. 2Key Laboratory of Xijiang Endemic and Ethnic Disease, Shihezi University, Shihezi 832003, China. 3College of Medicine, Shihezi University, Shihezi, 832003, China.
Email: [email protected]

  •  Accepted: 26 September 2011
  •  Published: 16 October 2011



This study aimed to investigate the role of autophagy in the inhibition of Brucella-containing vacuole (BCV) and lysosome fusion in Brucella suis 1330 infected murine macrophage RAW264.7 cells. Autophagosome induction was observed following B. suis 1330 infection. The autophagic pathway was inhibited pharmacologically by 3-methylademine (3-MA) treatment or by RNAi-mediated depletion of the autophagy related gene, LC3. Compared with controls, suppression of the autophagic pathway was associated with a significant decrease in B. suis 1330 internalization (P<0.05) and reduction in the detection of B. suis-containing vacuole and lysosome fusion by fluorescence colocalization. These results implicate autophagy in the inhibition of BCV-lysosome fusion which favors the survival of B. suis 1330 in murine macrophage RAW264.7 cells.


Key words: Brucella suis 1330, autophagy, BCV-lysosome fusion, colocalization.


Abbreviations: BCV, Brucella-containing vacuole; CFU, colony forming units; DMEM,Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum; GFP, green fluorescent protein; MOI, multiplicity of infection; MTT, 3-(4,5-Dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide; PBS, phosphate buffered saline; 3-MA, 3-methylademine; MDC,monodansylcadaverine; TRD, Texas red-dextran.