African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Biofilm formation ability of Paenibacillus polymyxa and Paenibacillus macerans and their inhibitory effect against tomato bacterial wilt

Bin Li1, Rongrong Yu2, Qiaomei Tang1, Ting Su1, Xiaoling Chen1, Bo Zhu1, Yanli Wang3, Guanlin Xie1 and Guochang Sun3*
  1State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China. 2Zhejiang University of Technology, Hangzhou 310032, China. 3State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Email: [email protected]

  •  Accepted: 17 October 2011
  •  Published: 09 November 2011

Abstract

 

The potential of biofilm formation of 16 Paenibacillus strains and their inhibitory effect against bacterial wilt of tomato seedlings were examined in this study. The crystal violet assay indicated that all strains of Paenibacillus except strain MB02-1202 formed biofilm after 96 and 144 h of incubation while there was not significant difference in biofilm formation between strains of Paenibacillus polymyxa and strains of Paenibacillus macerans. However, the increase level of biofilm formation was associated with the increase of the incubation time and the initial inoculum density. In addition, allPaenibacillus strains except strain MB02-428 reduced wilt incidence in tomato seedlings inoculated with Ralstonia solanacearum while the cell numbers of R. solanacearum inrhizosphere soil was reduced by all Paenibacillus strains compared to the pathogen control. In general, most strains of Paenibacillus were able to both form biofilm and protect tomato seedlings from bacterial wilt, indicating that biofilm formation may play an important role in the biocontrol of Paenibacillus. This is first study regarding the relationship between in vitro biofilm formation ability of Paenibacillus strains and their inhibitory activity against R. solanacearum.

 

Key words: Biofilm, PaenibacillusRalstonia solanacearum, tomato.