African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Isolation of a bacteriophage against Salmonella Dublin and determination of its physical resistance under varied in vitro conditions

Mudit Chandra1*, Sunita Thakur2, Deepti Narang3 and H. M. Saxena4
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana- 141004, India.
Email: [email protected]

  •  Accepted: 30 July 2011
  •  Published: 04 August 2011

Abstract

In the present study a phage against Salmonella Dublin was isolated using agar overlay technique. On physical characterization of the phage (pH, temperature and sun light exposure) it was found that the phage could survive at varied pH conditions with reduction in its numbers. A temperature of above 50°C and direct sunlight beyond 5 days was found to be deleterious for survival of the phage.

 

Key words: Salmonella, bacteriophage, physical parameters, pH, sunlight.