African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Laboratory evaluation of the entomopathogenic fungi, Iranian isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metsch) Sorokin against the control of the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Bruchidae)

Zahra Mahdneshin1, Samad Vojoudi*2, Youbert Ghosta1,  Mohammad Hassan Safaralizadae1 and Moosa Saber3
  1Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Iran. 2Young Researchers club, Marand Branch, Islamic Azad University, Marand, Iran.  3Department of Plant Protection, College of Agriculture, University of Maragheh, Maragheh, Iran.
Email: [email protected]

  •  Accepted: 18 November 2011
  •  Published: 09 December 2011



In this study, the pathogenicity of five Iranian isolates of Beauveria bassiana andMetarhizium anisopliae was evaluated against adults of Callosobruchus maculatus adults by immersion bioassay method at 27 ± 1°C and 60 ± 5% R.H. under laboratory conditions.Cumulative mortality of the cowpea weevil at day 11 after treatment with B. bassianaisolates IRAN 187C, IRAN 429C, IRAN 441C and M. anisopliae isolates IRAN 715C and DEM I001 by conidial suspensions at low and high concentration ranged between 29.4 to86.2, 9 to 88.3, 21.1 to 96.3, 24.8 to 84.4 and 20.9 to 80.9%, respectively. The LC50 and LT50 values for IRAN 187C, IRAN 429C, IRAN 441C, DEMI001 and IRAN 715C were 1.9×107, 9.8×106, 2.4×107, 2.6×10and 1.2×108 conidia/ml and 7.8, 6.7, 7.2, 7.7 and 7.8 day, respectively. IRAN 441C of B. bassiana had the highest virulence against adult cowpea weevil because it had lower LC50 and LT50 and caused the highest mortality (76%) in treatment by suspensions containing 1×108 conidia/ml. B. bassiana had higher virulence than M. anisopliae against adult of cowpea weevil.


Key word: Biological control, immersion bioassay, virulence, cowpea weevil.