African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Drug susceptibility test on Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in three sites of Ethiopia

Bedru Hussein3, Tewodros Debebe2*, Annelies Wilder-Smith4 and Gobena Ameni1  
1Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia. 2Department of Microbiology, Immunology and Parasitology, College of Medicine and Health Sciences, Bahir Dar University, P.O. Box 3040, Bahir Dar, Ethiopia. 3Department of Public Health, Mada Walabu University, P.O. Box 1176, Gobba, Ethiopia.4Institute of Public Health, Heidelberg University, Heidelburg, Germany
Email: [email protected]

  •  Accepted: 21 February 2013
  •  Published: 26 February 2013


The spread of multidrug-resistant (MDR) strains of Mycobacterium tuberculosis has become a challenge to the global tuberculosis control and prevention program.Nevertheless, no adequate information has been made available on the drug susceptibility status of M. tuberculosis in Ethiopia. Hence, this study aimed atevaluating the drug resistance patterns of M. tuberculosis in the three sites of Ethiopia and its association with socio-demographic factors. A total of 102 M. tuberculosis isolates obtained from tuberculosis (TB) patients from three different sites were tested for drug susceptibility against the first and second-line anti-TB drugs using the proportion method on enriched Middle Brook 7H10 agar. The result showed that 36.3% of the isolates were resistant to at least one of the first line anti-TB drugs used in this study. The proportions of mono-resistance were 29.4, 13.7, 13.7 and 23.5% to isoniazid, rifampicin, streptomycin and ethambutol, respectively. The proportion of MDR M. tuberculosis was 11.8%. Only 55% of the isolates were susceptible to all the second line anti-TB drugs used in this study. One isolate (1%) was extensively drug-resistant (XDR). The occurrence of MDR M. tuberculosisisolates was not associated with sex, age, anti-TB treatment history, HIV status andlocation. The findings of this study showed moderate number of MDR M. tuberculosis isolates in the study area, warranting the necessity of the surveillance and monitoring.


Key words: Mycobacterium tuberculosis, drug resistance, Ethiopia