African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

A novel assay combined PCR with junction probe for rapid detection of anaerobes DNA

Shuai Yan1, Li Zeng1*, Wenlin Li1 and Haihua Qian2
1Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ZIP: 210000. People’s Republic of China. 2Jiangsu Province Hospital of TCM, Nanjing, Jiangsu, ZIP: 210000. People’s Republic of China.
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 30 June 2013


Herein, we developed a novel assay for detection of anaerobes by polymerase chain reaction (PCR) combined with JP (junction probe). The PCR products were processed by bead-based extraction to get single strand DNA which could be used in the junction probe reaction. The experiment results demonstrate that the new assay which we call PCR-JP could detect at least 10 copies of DNA with high sensitivity and specificity which is easily operated in the elementary medical organizations and resource-limited areas.


Key words: Junction probe, PCR, Anaerobes, rapid detection.