African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Isolation and characterization of EST-SSRs in the Ramie

Jian-Hua Chen1, Ming-Bao Luan1, Shu-Feng Song2, Zi-Zheng Zou1, Xiao-Fei Wang1, Ying Xu1, and Zhi-Min Sun1
  1Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences / Key laboratory of Stem-fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha China.  2Institute of subtropical agricultural Chinese academy of science, Changsha, China.  
Email: [email protected]

  •  Accepted: 22 August 2011
  •  Published: 09 October 2011

Abstract

 

In order to develop the more EST-SSRs, 320 ESTs of ramie from NCBI were analyzed. 76 SSR primer-pairs developed from 320 ESTs were tested among 62 ramie individuals, and 27 SSR loci were polymorphic. Among the 27 primers, the numbers of alleles per locus ranged from two to five, in which 19 primers amplify two alleles, seven primers amplify three alleles, and only one primer amplifies five alleles. Observed heterozygosity (HO) of overall loci among the 27 primer pairs ranged from 0.16 to 0.93 and expected heterozygosity(HE) ranged from 0.21 to 0.66.Of the 27 loci, 14 displayed significant deviations from Hardy–Weinberg expectations (P < 0.05). No significant linkage disequilibrium (LD) was detected among the 27 loci. The 27 primers have been used in the ramie molecular linkage map and genetic diversity analysis successfully.

 

Key words: Microsatellite marker, ramie, EST, EST-SSR.